Incidence of Oxa23 and Oxa51 Genes Associated with Bacterial Isolated from Patients with Urosepsis: Single Centre Prespective

Background: Urosepsis is one of the most common infections that require empirical broad spectrum antibiotics immediately after diagnosis. This has led to development bacterial resistance by acquiring capability destroy β-lactam ring. Methodology: a cross-sectional hospital-based study. The study was conducted from 2019 2020 at Gezira Hospital for Renal diseases and surgery (GHRDS). A hundred patients were diagnosed clinically with urosepsis isolated organisms Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumonia Pseudomonas aeruginosa. susceptibility test Kirby Bauer disc diffusion technique according clinical laboratory standard institute (CLSI) guidelines. Seventy eight samples genomic DNA confirmed 16srRNA multiplex PCR, performed genotypic blaOXA-51 blaOXA-23 gene characterization bacteria. Then gel electrophoresis used identify presence or absence (blaOXA-51 blaOXA-23) genes. Results: 88.5% (69/78) in detected. Using frequencies genes 13% 10.1%, respectively. percentages isolates which yielded both among P. aeruginosa 25% (1/4), K. 17% (1/6), E. coli 8% (3/37). Only detected mirabilis 10% (1/10) only S. aureus 5% (1/18). Conclusion: In this study, increased